Applying a potential across a biomembrane: electrostatic contribution to the bending rigidity and membrane instability

We investigate the effect on biomembrane mechanical properties due to the presence an external potential for a non-conductive non-compressible membrane surrounded by different electrolytes. By solving the Debye-Huckel and Laplace equations for the electrostatic potential and using the relevant stress-tensor we find: in (1.) the small screening length limit, where the Debye screening length is smaller than the distance between the electrodes, the screening certifies that all electrostatic interactions are short-range and the major effect of the applied potential is to decrease the membrane tension and increase the bending rigidity; explicit expressions for electrostatic contribution to the tension and bending rigidity are derived as a function of the applied potential, the Debye screening lengths and the dielectric constants of the membrane and the solvents. For sufficiently large voltages the negative contribution to the tension is expected to cause a membrane stretching instability. For (2.) the dielectric limit, i.e. no salt (and small wavevectors compared to the distance between the electrodes), when the dielectric constant on the two sides are different the applied potential induces an effective (unscreened) membrane charge density, whose long-range interaction is expected to lead to a membrane undulation instability.Comment: 16 pages, 3 figures, some revisio

Prospecting for organic fungicides and resistance inducers to control scab (Venturia inaequalis) in organic apple production

As a part of the REPCO project we are prospecting for plant extracts and other materials to control apple scab in order to find substitutes for copper fungicides in organic apple production. Two routine screening systems are used to evaluate the potential of materials to control apple scab; one testing the effect of materials on conidium germination on glass slides, and one testing the effect of materials on scab development on apple seedlings grown in growth chambers. Several interesting materials have been identified and a number have been selected for testing in experimental organic apple orchards. Studies of the mode of action of promising materials including induced resistance are on-going and work on improvement of timing and formulation is initiated at KVL

Osmotic properties of polyethyleneglycols: quantitative features of brush and bulk scaling laws

From glycosylated cell surfaces to sterically stabilized liposomes, polymers attached to membranes attract biological and therapeutic interest. Can the scaling laws of polymer "brushes" describe the physical properties of these coats? We delineate conditions where the Alexander - de Gennes theory of polymer brushes successfully describes the intermembrane distance vs. applied osmotic stress data of Kenworthy et al. for PEG-grafted multilamellar liposomes [Biophys. J. (1995) 68:1921]. We establish that the polymer density and size in the brush must be high enough that, in a bulk solution of equivalent density, the polymer osmotic pressure is independent of polymer molecular weight (the des Cloizeaux semi-dilute regime of bulk polymer solutions). The condition that attached polymers behave as semi-dilute bulk solutions offers a rigorous criterion for brush scaling-law behavior. There is a deep connection between the behaviors of polymer solutions in bulk and polymers grafted to a surface at a density such that neighbors pack to form a uniform brush. In this regime, two-parameter unconstrained fits of the Alexander - de Gennes brush scaling laws yield effective monomer lengths of 3.3 to 3.5 AA, which agree with structural predictions. The fitted distances between grafting sites are larger than expected from the nominal content of PEG-lipids; the chains apparently saturate the surface. Osmotic stress measurements can be used to estimate the actual densities of membrane-grafted polymers.Comment: 26 pages with figure

Third Party Tracking in the Mobile Ecosystem

Third party tracking allows companies to identify users and track their behaviour across multiple digital services. This paper presents an empirical study of the prevalence of third-party trackers on 959,000 apps from the US and UK Google Play stores. We find that most apps contain third party tracking, and the distribution of trackers is long-tailed with several highly dominant trackers accounting for a large portion of the coverage. The extent of tracking also differs between categories of apps; in particular, news apps and apps targeted at children appear to be amongst the worst in terms of the number of third party trackers associated with them. Third party tracking is also revealed to be a highly trans-national phenomenon, with many trackers operating in jurisdictions outside the EU. Based on these findings, we draw out some significant legal compliance challenges facing the tracking industry.Comment: Corrected missing company info (Linkedin owned by Microsoft). Figures for Microsoft and Linkedin re-calculated and added to Table